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Chroma Technology (filter/spectra information)

System Fluorophore (recommendations)

Fluorophores/Dyes

Fluorophore/Dye Spectra
Name                     
Excitation 1
Excitation 2
Emission 1
Emission 2
4 Metylumbelliferon
360
450
6 Aminoquinolin
360
443
7 Amino Actinomycin D
546
647
Acridingelb               
470
550
Acridine orange (+ DNA)
502
526
Acridine orange (+RNA)
460
650
Acriflavin-Feulgen
480
550-600
Alexa 350
343
440
Alexa 430
430
540
Alexa 488 
491
515
Alexa 532
523
548
Alexa 546
553
569
Alexa 568
573
596
Alexa 594
585
610
Alizarinkcomplexion
530-560
580
Allophycocyannin
630
660
AMCA
345
440-456
Amino-Methylcumarin
354
441
Atebrin
436
490
Auramin
460
550
Auraphospin
450-490
515
B-Phycoerythrin
546
565
575
565
BAO
365
395
BCECF
430
480
520
480
Berberinsulfat
430
550
BFP (Blue-shifted/Y66H)
380
440
BFP2
380
440
Blue Fluospheres
360
415
BOBO-1
462
481
BOBO-3
570
602
BODIPY
503
512
BODIPY 581/591 phalloidin
584
592
C-Phycocyanin
605
645
Calcein
495
520-550
Calcein Blue
360
440-450
Calcium Crimson Indicator
590
615
Calcium Green
506
533
Calcium Orange
554
576
Calcofluor White
440
510
Cascade Blue
400
420
Catecholamine
410
470
CFP(“cyan”/Y66W)
433
453
475
453
Chinacrin
450-490
515
Chromomycin/Mitramycin
436-460
470
Coriphosphin
460
575
CPM
385
471
Crimson Fluospheres
625
645
CTC(5-cyano2,3ditolylTetrazolium Chlorid)
602
111
Cyanine 3 Cy 3
553
575
Cyanine 5 Cy 5
651
674
Cyanine 7 Cy 7
710
805
Dansylchlorid
380
475
DAO DIAMINO Phenyl Oxyd
470
610
DAPI + DNA
359
461
Dark Red FluoSpheres
660
680
dE GFP
488
509
Diaminonaphtylsulfonsaure
340
525
DiBac4(Dibutylbarbitursaure Trimethinoxonol)
493
516
DIDS Thiocyanatostilbene
342
418
DilC3/4/12/22 Indocarbocyanine    
540-560
556-575
DiOC7/16/2 Carbocyanine
550
580
DiSC1/2/6 Thiocarbocyanine
559
585
Dopamin
340
490-520
DTAF
495
528
EBFP
380
440
ECFP
443
453
475
453
EGFP
488
509
EOSIN
524
544
Ethidium bromide (+DNA)
510
595
Euchrysin
430
540
Evans blue
550
611
EYFP
514
488
527
488
Fuelgen
480
560
FIF-Falk
Fluo-3
480
520
Fluorescamin FLURAM
Fluoresceindiacetat FDA
499
Fluoresceinisothiocyanat FITC
490
525
Fluorogold
350-395
530-600
FURA-2
340
363
500
363
Generic blue/green combination
GFP (AAV)
395
470
510
470
GFP (ASV)
395
470
510
470
GFP (LVA)
395
470
510
470
GFP-S65T (red shifted)
488
509
GFP mut3.1
395
470
510
470
GFP UV
360-400
510
Haematoporphyrin
530-560
580
Hoechst 34580(Bisbenzimid)
392
440
Hoechst 33342(Bisbenzimid)
352
461
Indo-1
360
410
Lissamin-Rodamine B
574
602
Lucifer Yellow
428
540
Magdalrot
524
600
Mepacrin
Merocyanin
555
578
Methylgrun/Pyronin Stilben
364
395
Mithramycin
420
575
NBD-Amine
460-485
534-542
NBD-Chlorid
480
510-545
Nil Rot
549
628
Noradrenalin
340
490-520
Olivomycin
340-480
470-630
Oxytetracyclin
Pararosanilin-Feulgen
560
625
PBFI
340
380
557
380
Phosphin 3R
465
565
POPO-1
434
456
POPO-3
534
570
Primulin
410
450
Propidium iodide
536
617
Pyren
343
380-400
Pyronin
490-580
530-610
QUIN 2
340
365
490
365
Quinacrine mustard
423
510
R-Phycoerythrin
480-550
578
R-Phycolrythrin
565
575
Red Fluorospheres
580
605
Resorufin
571
585
Rhodamine
540-507
529
Rhodamine 123
540-560
580
Rhodamine phalloidin
550
575
Saurefuchsin, Fuchsinsaeure
540
630
SBFI
340
380
420
380
Sertonin
365
520
SNARF
480
600
Stilben SITS, SITA
365
460
Sulfaflavin
380-470
470-580
Tetracycline
390
560
Tetrametylrhodamine
540
566
Texas Red
595
620
Thiazinrot
510
580
Thioflavin S
430
550
TOTO-1 + DNA
509
533
TOTO-3 + DNA
642
661
TRITC
520-554
580
wtGFP
395
470
510
470
XRITC
572
596
Yellow-Green Fluospheres
505
515
YFP(Au yellow‚ Au T203Y)
513
488
527
488
YOYO-1
491
509
YOYO-3
612
631