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Protocols

 

Light Microscopy
Electron Microscopy
   
• Acid Phosphatase • Acid Phosphatase
• Antibody Purification • Alkaline Phosphatase
• Eisen_PBS • Animal perfusion
• FA Fixation for IF • Au-A-PA preparation
• Gelatin Coating Slides • Colloidal Gold
• Glycogen Staining • Drosophila ImmunoEM
• IF on Cells with Avidin/Biotin • Drodophila Morphology
• IF on Semi-thin Cryosections • EISEN_PBS
• Immunocytochemistry on Cultured Cells • EM Fractions
• In Situ Hybridization • FA Fixation for ImmunoEM
• Immunoperoxidase on Cells • Formvar Grids
• PBS • Gelatin Coated Coverslips
• Yeast Immunofluorescence • Immunogold Labeling of Ultrathins
  • Immunodiffusion
  • In Situ Hybridization
  • Immunoperoxidase on Cells
  • Kleinschmidt Technique
Methods Papers
• Yeast KMnO4
• McCaffery and Farquhar, 1994. Immunolocalization of GTPases • Yeast OTO
• Perkins and McCaffery, 2007. EM/IEM of Mitochondria • LR White Processing
  • Metal Shadowing
  • Neutral UA
  • PBS
  • PLP Fixation (Nakane and McLean)
  • Protein A Gold
  • Routine Morphology