1. Lai SK, Hida K, Chen C, Hanes J. Characterization of the intracellular dynamics of a non-degradative pathway accessed by polymer nanoparticles.
   J Control Release. 2008 Jan 22;125(2):107-11. [Pubmed] [PDF]
   
   
2. Lai SK, Hida K, Man ST, Chen C, Machamer C, Schroer TA, Hanes J. Privileged Delivery of Polymer Nanoparticles to the Perinuclear Region of Live Cells via a Non-Clathrin, Non-Degradative Pathway.
   Biomaterials. 2007 Jun;28(18):2876-84. [Pubmed] [PDF]
   
   
3. Suk JS, Suh J, Lai SK, Hanes J. Quantifying the intracellular transport of viral and nonviral gene vectors in primary neurons.                                                                                                                                                                                                    Exp Biol Med (Maywood). 2007 Mar;232(3):461-9.  [Pubmed]
   
   
4. Lai SK, O'Hanlon ED, Harrold S, Man ST, Wang YY, Cone R, Hanes J. Rapid transport of large polymeric nanoparticles in fresh undiluted human mucus.
   PNAS, Proc Natl Acad Sci. 2007 Jan 30;104(5):1482-1487. [Pubmed] [PDF]
   [Research highlights, "Nanoparticles: Against the flow", Nature Nanotechnology (2007) [Link] ; Research highlights, JAMA (Journal of American Medical Association) March 2007; Research highlights,"Coated Nanoparticles Solve Sticky Drug-Delivery Problem" Nanotech News, NCI, February 2007 [Link] ; also featured in over 30 science/medical magazines and newspapers, including The New Scientist. [Link] ] 
   
   
5. Lai SK, Hanes J. Real time multiple particle tracking of gene nanocarriers in complex biological environments.                                                                                                                                                                                        In Gene Therapy Protocol, 2007; J Ledoux (Ed.), 3rd Ed. The Humana Press, Totowa.
                                               
6. Suk JS, Suh J, Choy K, Lai SK, Fu J, Hanes J. Gene delivery to differentiated neurotypic cells with RGD and HIV Tat peptide functionalized polymeric nanoparticles.
   Biomaterials. 2006 Oct; 27(29):5143-5150 [Pubmed] [PDF]
   [Research highlights, "Enhancing gene delivery with nanoparticles could ultimately lead to a cure for Alzheimer's", Nanowerk (2006) [Link] ; Research highlights, "2006 top 20 viewed paper in Biomaterials" (2006) ; Research highlights, "SEBM Best Paper Award for 2007 in the experimental biology/basic research category."] 
   
   
7. Fiegel J, Jin F, Hanes J, Stebe K. Wetting of a particle in a thin film.
   J Colloid Interface Sci. 2005 Nov;291(2):507-14 [Pubmed] [PDF]
   
   
8. Sakhalkar HS, Hanes J, Fu J, Benavides U, Malgor R, Borruso CL, Kohn LD, Kurjiaka DT, Goetz DJ. Enhanced adhesion of ligand-conjugated biodegradable particles to colitic venules.
   FASEB J. 2005 May;19(7):792-4 [Pubmed] [PDF]
   
   
9. Suh J, Dawson M, Hanes J. Real-time multiple-particle tracking: applications to drug and gene delivery.
   Adv Drug Deliv Rev. 2005 Jan;57(1):63-78. [Pubmed] [PDF]
   
   
10. Dawson M, Krauland E, Wirtz D, Hanes J. Transport of polymeric nanoparticle gene carriers in gastric mucus.
    Biotechnol Prog. 2004 May-Jun;20(3):851-7. [Pubmed] [PDF]
    
    
11. Fiegel J, Fu J, Hanes J. Poly(ether-anhydride) dry powder aerosols for sustained drug delivery in the lungs.
    J Control Release. 2004 May;96(3):411-23. [Pubmed] [PDF]
    
    
12. Suh J, Wirtz D, Hanes J. Real-time intracellular transport of gene nanocarriers studied by multiple particle tracking.
    Biotechnol Prog. 2004 Mar-Apr;20(2):598-602. [Pubmed] [PDF]
    
    
13. Li H, Yuan Z, Lam KY, Lee HP, Chen J, Hanes J, Fu J. Model development and numerical simulation of electric-stimulus-responsive hydrogels subject to an externally applied electric field.
    Biosens Bioelectron. 2004 Apr;19(9):1097-107. [Pubmed] [PDF]
    
    
14. Li H, Yuan Z, Ng TY, Lee HP, Lam KY, Wang QX, Wu S, Fu J, Hanes J. Constitutive model development and micro-structural topology optimisation for nafion hydrogel membranes with ionic clustering.
    J Biomater Sci Polym Ed. 2003;14(11):1181-96. [Pubmed] [PDF]
    
    
15. Sakhalkar HS, Dalal MK, Salem AK, Ansari R, Fu J, Kiani MF, Kurjiaka DT, Hanes J, Shakesheff KM, Goetz DJ. Leukocyte-inspired biodegradable particles that selectively and avidly adhere to inflamed endothelium in vitro and in vivo.
    Proc Natl Acad Sci U S A. 2003 Dec;100(26):15895-900. [Pubmed] [PDF]
    
    
16. Dawson M, Wirtz D, Hanes J. Enhanced viscoelasticity of human cystic fibrotic sputum correlates with increasing microheterogeneity in particle transport.
    J Biol Chem. 2003 Dec;278(50):50393-401. [Pubmed] [PDF]
    
    
17. Suh J, Wirtz D., Hanes J. Efficient Active Transport of Gene Nanocarriers to the Cell Nucleus.
    Proc Natl Acad Sci U S A. 2003 Apr;100(7):3878-82 [Pubmed] [PDF]
    
    
18. Fiegel J, Ehrhardt C, Schäfer U, Lehr C-M, Hanes J. Large Porous Particle Impingement on Lung Epithelial Cell Monolayers: Toward Improved Particle Characterization in the Lung,
    Pharm. Res. 2003 May;20(5):788-96. [Pubmed] [PDF]
    
    
19. Hanes J, Dawson M, Har-el Y, Suh J, Fiegel J (2003) Gene delivery to the lung. In: AJ Hickey (Ed.) Pharmaceutical Inhalation Aerosol Technology, 2nd Ed. New York: Marcel Dekker Inc.: New York, 489-539. [PDF]
    
    
20. Rhines, L.D., P. Sampath, F. Dimeco, H.C. Lawson, B.M. Tyler, Y. Har-el, J. Hanes, A. Olivi, D.M. Pardoll, H. Brem. Paracrine immunotherapy with interleukin-2 delivered via biodegradable polymer microspheres combined with interstitial chemotherapy: A novel treatment for experimental malignant glioma.
    Neurosurgery 2003 Apr; 52:872-880, 2003. [Pubmed] [PDF]
    
    
21. 5. Fu, J., Krauland, E., Har-el, Y., and Hanes, J. (2002) New degradable cationic polyesters for nonviral gene delivery,
    Proceedings of the Second Joint EMBS/BMES Conference, 551-2.
    
    
22. van Zanten, J. H., Har-el, Y., and Hanes, J. (2002) Optimization of PEI/VEGF DNA polyplexes for potential delivery from tissue engineering scaffolds,
    Proceedings of the Second Joint EMBS/BMES Conference, 563-4.
    
    
23. Suh, J., Wirtz, D., and Hanes, J. (2002) Real-time Quantitation of Intracellular Polycation/DNA Nanocomplex Transport: Evidence for Directed Motion, Proceedings of the Second Joint EMBS/BMES Conference, 548-9.
    
    
24. Fu J, Fiegel J, Krauland E, Hanes J. New polymeric carriers for controlled drug delivery following inhalation or injection,
    Biomaterials 2002 Nov; 23: 4425-4433. [Pubmed] [PDF]
    
    
25. Ehrhardt C, Fiegel J, Fuchs S, Abu-Dahab R, Schäfer UF, Hanes J, Lehr C-M. Drug absorption by the respiratory mucosa: Cell culture models and particulate drug carriers.
    J. Aerosol Med. 2002 Summer; 15:131-140. [Pubmed] [PDF]
    
    
26. Ehrhardt C, Kneuer C., Fiegel J, Hanes J, Schäfer UF, Kim K.J., Lehr C-M. Influence of apical fluid volume on the development of functional cellular junctions in the human epithelial cell line 16HBE14o-: Implications for its use as an in vitro model for bronchial drug absorption studies.
    Cell & Tissue Res. 2002 Jun; 308: 391-400. [Pubmed] [PDF]
    
    
27. Hanes J, Sills AK, Zhao Z, Suh KW, Tyler BM, DiMeco F, Brat DJ, Choti MC, Leong KW, Pardoll DM, Brem H. Controlled local delivery of interleukin-2 by biodegradable polymers protects animals from experimental brain tumors and liver tumors.
    Pharm. Res. 2001 Jul; 18:899-906. [Pubmed] [PDF]
    
    
28. Har-el, Y., van Zanten, J. H., and Hanes, J. (2001) Polycation/DNA nanocomplex delivery from porous PLGA microspheres, Annals of Biomedical Engineering. 29: S-96.
    
    
29. Hanes J, Fiegel J, Fu J (2001) New polymers for controlled pulmonary drug delivery, Annals of Biomedical Engineering. 29: S-138.
    
    
30. Fiegel, J., Ehrhardt C, Lehr C-M, Hanes J (2001) Air-interfaced lung epithelial cell monolayers for characterization of large light polymer aerosols. Annals of Biomedical Engineering, 29: S-141.
    
    
31. DiMeco, F., Rhines, L. D., Hanes, J., Tyler, B. M., Brat, D., Torchiana, E., Guarnieri, M., Colombo, M. P., Pardoll, D. M., Finocchiaro, G., Brem, H., Olivi, A. Paracrine delivery of IL-12 against intracranial 9L gliosarcoma in rats.
    Journal of Neurosurgery 2000 Mar; 92: 419-427. [Pubmed] [PDF]
    
    
32. Ewend, M. G., Thompson, R. C., Anderson, R., Sills, A. K., Staveley-O'Carroll, K., Tyler, B. M., Hanes, J., Brat, D., Thomas, M., Jaffee, E. M., Pardoll, D. M., and Brem, H. Intracranial paracrine interleukin-2 therapy stimulates prolonged antitumor immunity that extends outside the central nervous system.
    Journal of Immunotherapy 2000 Jul-Aug; 23: 438-448. [Pubmed] [PDF]
    
    
33. Sampath P, Hanes J, DiMeco F, Tyler BM, Brat D, Pardoll DM, Brem H. Paracrine immunotherapy with interleukin-2 and local chemotherapy is synergistic in the treatment of experimental brain tumors.
    Cancer Research 1999 May; 59:2107-2114. [Pubmed] [PDF]
    
    
34. Hanes, J., Chiba, M., Langer, R. Degradation of porous poly(anhydride-co-imide) microspheres and implications for controlled macromolecule delivery,
    Biomaterials 1998 Jan-Feb; 19:163-172. [Pubmed] [PDF]
    
    
35. Evora C., Soriano I., Rogers R., Shakesheff K.M., Hanes J., Langer R. Relating the phagocytosis of microparticles by alveolar macrophages to surface chemistry: The effect of 1,2-dipalmitoylphosphatidylcholine.
    J. Control Release. 1998 Feb , 51:143-152. [Pubmed] [PDF]
    
    
36. Batycky R.P., Hanes J., Langer R., Edwards D.A. Theoretical model of erosion and macromolecular drug release from biodegrading microspheres.
    J. Pharm. Sci. 1997 Dec; 86:1464-1477. [Pubmed] [PDF]
    
    
37. Hanes J., Cleland J.L., Langer R. New advances in microsphere-based single-dose vaccines.
    Adv. Drug Del. Rev. 1997 Oct; 28:97-119. [Pubmed] [PDF]
    
    
38. Edwards, D. A., Hanes, J., Caponetti, G., Hrkach, J., Ben-Jebria, A., Eskew, M. L., Mintzes, J., Deaver, D., Lotan, N., and Langer, R. Large porous particles for pulmonary drug delivery.
    Science 1997 Jun; 276: 1868-71. [Pubmed] [PDF]
    
    
39. Chiba, M., Hanes, J., and Langer, R. Controlled protein delivery from biodegradable tyrosine-containing poly(anhydride-co-imide) microspheres.
    Biomaterials 1997 Jul; 18: 893-901. [Pubmed] [PDF]
    
    
40. Hanes, J. and Burke, M. (1997) Polymer-controlled drug delivery: An overview for the clinician, Hospital Pharmacist Report (December Issue Supplement): 1-11.
    
    
41. Hanes J., Chiba M., Langer R. (1996) Synthesis and characterization of degradable anhydride-co-imide terpolymers containing trimellitylimido-L-tyrosine: Novel polymers for drug delivery, Macromolecules 29:5279-5287. [PDF]
    
    
42. Hanes J., Langer R. (1996) Polymeric controlled release vaccine delivery systems, in: Reproductive Immunology, (R. Bronson, N. Alexander, D. Anderson, D.W. Branch and W.H. Kutteh eds.), Blackwell Scientific Publications, Inc., Boston, pp. 647-664.
    
    
43. Kline D.F., Hanes J., Langer R. (1996) Adjuvant-active polymeric microparticulate vaccine-delivery systems, in: Microparticulate Systems for the Delivery of Proteins and Vaccines, (S. Cohen and H. Bernstein eds.), Marcel Dekker, Inc., New York, pp. 349-380.
    
    
44. Hanes J., Chiba M., Langer R. (1995) Polymer microspheres for vaccine delivery, in: Vaccine Design: The Subunit and Adjuvant Approach, (M.F. Powell and M.J. Newman eds.), Plenum Press, New York, pp. 389-412. [Pubmed]
    
    

Peer-Reviewed Extended Abstracts

1. Wei C, Lyubchenko YL, Ghandehari H, Hanes J, Stebe KJ, Mao HQ, Haynie DT, Tomalia DA, Foldvari M, Monteiro-Riviere N, Simeonova P, Nie S, Mori H, Gilbert SP, Needham D; American Academy of Nanomedicine. New technology and clinical applications of nanomedicine: highlights of the second annual meeting of the American Academy of Nanomedicine (Part I). Nanomedicine. 2006 Dec;2(4):253-63. [Pubmed]
   
   
2. Sakhalkar HS, Salem A, Shakesheff KM, Morita T, Hanes J, Ansari R, Kiani MF, Goetz DJ. Targeting Biodegradable Particles to Select Segments of the Endothelium in Vitro and in Vivo, Proceedings of the Controlled Release Society Winter Symposia; Salt Lake City, UT, March 2003.
   
   
3. Ehrhardt, C., Fiegel, J., Schaefer, U. F., Hanes, J., and Lehr, C. M. Calu-3 bronchial epithelial cell monolayers to study drug release and absorption from aerosol particles in vitro, Proceedings of the 4th World Meeting ADRITELF/APGI/APV 1009-1010, 2002.
   
   
4. Fu, J., Krauland, E., Har-el, Y., and Hanes, J. New degradable cationic polyesters for nonviral gene delivery, Annals of Biomedical Engineering, 2002.
   
   
5. Suh, J., Wirtz, D., and Hanes, J. Real-time Quantitation of Intracellular Polycation/DNA Nanocomplex Transport: Evidence for Directed Motion, Annals of Biomedical Engineering, 2002. (Invited)
   
   
6. van Zanten, J. H., Har-el, Y., and Hanes, J. Optimization of PEI/VEGF DNA polyplexes for potential delivery from tissue engineering scaffolds, Annals of Biomedical Engineering, 2002.
   
   
7. Fiegel, J., Ehrhardt, C., Lehr, C. M., and Hanes, J. Air-interfaced lung epithelial cell monolayers for characterization of large light polymer aerosols, Annals of Biomedical Engineering. 29: S-141, 2001.
   
   
8. Fu, J., Fiegel, J., and Hanes, J. Large, light polyether-anhydride microspheres: A new carrier for pulmonary drug delivery, Proceedings of the International Symposium on Controlled Release of Bioactive Materials. Controlled Release Society, San Diego, CA, 28: 393-394, 2001.
   
   
9. Hanes, J., Fiegel, J., and Fu, J. New polymers for controlled pulmonary drug delivery, Annals of Biomedical Engineering. 29: S-138, 2001.
   
   
10. Har-el, Y., Janardhana, D., and Hanes, J. Enhanced transection efficiency through long term delivery of PEI/DNA nanocomplexes from porous PLGA microspheres, Proceedings of the International Symposium of Controlled Release and Bioactive Matierals. Controlled Release Society, San Diego, CA, 28: 1213-1214, 2001.
    
    
11. Har-el, Y., van Zanten, J. H., and Hanes, J. Polycation/DNA nanocomplex delivery from porous PLGA microspheres, Annals of Biomedical Engineering. 29: S-96, 2001.
    
    
12. Tam, J., Fiegel, J., and Hanes, J. Protein crystals for pulmonary controlled drug delivery, Proceedings of the International Symposium on Controlled Release of Bioactive Materials. Controlled Release Society, San Diego, CA, 28: 1027-1028, 2001.
    
    
13. Hanes J., Suh K., Tyler B., DiMeco F., Brat D., Choti M., Leong K., Pardoll D., Brem, H., Local interleukin-2 delivery eradicates brain and liver tumors, Proc. International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society, Las Vegas, NV, 25:253-254, 1998.
    
    
14. Hanes, J., Evora, C., Ben-Jebria, A., Edwards, D. A., and Langer, R. Porous dry-powder PLGA microspheres coated with lung-surfactant for systemic insulin delivery via the lung, Proceedings of the 24th International Symposium on Controlled Release of Bioactive Materials. Controlled Release Society, Stockholm, Sweden, 24: 57-58, 1997.
    
    
15. Hanes, J., Hildgen, P., Batycky, R. P., Cleland, J. L., Edwards, D. A., and Langer, R. Porous poly(D,L-lactic-co-glycolic acid) microsphere degradation and release of macromolecules, Proceedings of the 24th International Symposium on Controlled Release of Bioactive Materials. Controlled Release Society, Stockholm, Sweden, 24: 1027-1028, 1997.
    
    
16. Edwards, D.A., Caponetti, G., Hrkach, J., Hanes, J., Lotan, N., Ben-Jebria, A., and Langer, R., Large porous aerosols for pulmonary drug delivery, in: Proceedings of the 24th International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society, Stockholm, Sweden, 24:67-68, 1997.
    
    
17. Batycky, R.P., Hanes, J., Langer, R., and Edwards, D.A., A theory of erosion and macromolecule release from biodegrading microspheres, in: Proceedings of the 24th International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society, Stockholm, Sweden, 24:167-168, 1997.
    
    
18. Hanes, J., Chiba, M. and Langer, R., Degradation and Erosion of Tyrosine-Containing Polyanhydrides: Implications for Vaccine Delivery, in: Proceedings of the 21st International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society, Nice, France, 21:44-45, 1994.
    
    
19. Chiba, M., Hanes, J. and Langer, R., Polyanhydride microspheres as potential vaccine delivery systems, in: Proceedings of the 21st International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society, Nice, France, 21:877-878, 1994.

Patents

1. Edwards, D.A., Caponetti, G., Hrkach, J.S., Lotan, N., Hanes, J., Langer, R., Ben-Jebria, A. Amorphous porous particles for deep lung delivery, U.S. Patent No. 6,447,752. Granted September 10, 2002. (Continuation of U.S. Patent No. 6,254,854)
   
   
2. Edwards, D.A., Caponetti, G., Hrkach, J.S., Lotan, N., Hanes, J., Langer, R., Ben-Jebria, A. Porous particles for deep lung delivery, U.S. Patent No. 6,447,753. Granted September 10, 2002. (Continuation of U.S. Patent No. 6,254,854)
   
   
3. Edwards, D.A., Caponetti, G., Hrkach, J.S., Lotan, N., Hanes, J., Langer, R., Ben-Jebria, A. Porous particles for deep lung delivery, U.S. Patent No. 6,254,854. Granted July 3, 2001.
   
   
4. Hanes, J., Edwards, D.A., Evora, C., and Langer, R., Particles incorporating surfactants for pulmonary drug delivery, U.S. Patent RE37,053. Granted February 13, 2001. Continuation of U.S. Patent No. 5,855,913.
   
   
5. Edwards, D.A., Caponetti, G., Hrkach, J.S., Lotan, N., Hanes, J., Ben-Jebria, A.A., and Langer, R., Aerodynamically light particles for pulmonary drug delivery, U.S. Patent No. 6,136,295. Granted October 24, 2000. (Divisional of U.S. Patent No. 5,874,064)
   
   
6. Edwards, D.A., Caponetti, G., Hrkach, J.S., Lotan, N., Hanes, J., Ben-Jebria, A.A., and Langer, R., Aerodynamically light particles for pulmonary drug delivery, U.S. Patent No. 5,874,064. Granted February 23, 1999.
   
   
7. Hanes, J., Edwards, D.A., Evora, C., and Langer, R., Particles incorporating surfactants for pulmonary drug delivery, U.S. Patent No. 5,855,913. Granted January 5, 1999.
   
   
8. Hanes J, Fiegel J, and Fu J, Biodegradable polymer compositions: Compositions and uses related thereto. (Pending)
   
   
9. Edwards, D.A., Hanes, J., Evora, C., Langer, R., Vanbever, R., Mintzes, J., Wang, J., Chen, D., Preparation of Particles for Inhalation. (Pending)
   
   
10. Edwards, D.A., Caponetti, G., Hrkach, J.S., Lotan, N., Hanes, J., Ben-Jebria, A.A., and Langer, R., Aerodynamically light particles for pulmonary drug delivery, (Pending) Continuation of U.S. Patent Application 09/562,988, filed May 1, 2000, which is a continuation of U.S. Patent No. 6,136,295.