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General Information

    Fueled by the ever-growing DNA sequence information, proteomics, the large-scale analysis of proteins, has become one of the most important disciplines to characterize protein activities and provide insight into functional networks between protein molecules in a high-throughput format. Emerging evidence has pointed out that proteins rarely act as single, isolated species when performing their functions in vivo; they normally associate with other proteins and/or molecules as complexes and function in a network mode. Currently, my laboratory is focused to characterize the activities of large collection of proteins, build signaling networks for better understanding the mechanisms of biological processes, and identify biomarkers in human diseases and cancers. More specifically, our group is interested in analyzing protein posttranslational modifications, identifying important components involved in transcription networks and host-pathogen interactions on the proteomics level, and biomarkers in human IBD diseases.
    To achieve the above goals we have streamlined protein production in a high-throughput format that allows us to purify >2,000 proteins per day from both prokaryotic and eukaryotic hosts, managed to fabricate protein chips at high-density and high speed, and established a variety of biochemical assay protocols for the analysis of protein function at the proteome level.
    Using these technologies, we have fabricated a handful proteome chips in viruses, bacteria, yeast, and humans. Using our assay protocols, we have shown that protein chips can serve as a versatile tool to identify binding activities of proteins, including protein-protein, protein-nucleic acid, protein-lipid, protein-DNA/RNA, and protein-drug interactions. The enormous potential of the protein chip technology is also reflected by our recent studies on profiling human immunoresponses and identification of downstream substrates of protein kinases, acetylases, and ubiquitin E3 ligases.

Contact Information:
Heng Zhu
E-mail: hzhu4@jhmi.edu
Phone: (410) 502-0878
FAX: (410) 502-1872