NMR manager
Johns Hopkins School of Medicine
312 WBSB
Department of Pharmacology
725 N Wolfe St
Baltimore, MD 21205
410-502-4803(office)
Our department has one 400MHz auto switchable varian
Mercury spectrometer, one 500MHz Varian Inova spectrometer and one Inova
600MHz spectrometer with cryoprobe The 400MHz spectrometer is open to
all users for running experiments on small molecules. It has two
channels. The high band channel is for the detection of proton and fluorine.
The low band channel is for detection of carbon and phosphorus.
For Bio-molecular NMR, protein-DNA interactions, please contact
Prof. Jim Stivers. Dr. Ananya Majumdar,
director of bio-NMR center of JHU,
is ready to help you set up any kind of advanced experiments on our 600
MHz spectrometer and the 800MHz at Homewood center as well. Contact
information: email:
ananya@jhu.edu Tel:(410) 516 8670 (Office).
You need to be trained before running any NMR experiments: Our 400MHz machine can run PRESAT, and a number of 2D experiments such as NOESY, TOCSY and DEPT, HMBC and HMQC.
Please contact me for training on Mercury 400 NMR Training Guide.
Preparing your NMR sample:
Ideally, your sample should be dissolved in 0.7mL of deuterated solvent. Use
good quality NMR tubes (i.e.
(http://www.wilmad.labglass.com/category/2008; for routine use cat# WG-1228-7;
for use on 500-600 MHz
NMR cat#527-PP-7 ). Before inserting your sample into the probe, you MUST use
the sample depth gauge to
set the NMR tube at the right depth in the spinner. The depth gauge should be
set at 65mm and should not
be adjusted. DO NOT CHANGE THE SETTING OF THE DEPTH GAUGE. Avoid touching the
black strip at
the top of the spinner as you insert your sample (see instructions for basic
proton 1D on the next page).