TECHNIQUES and METHODOLOGIES

• Remote, web-based imaging is available for all LM/EM applications (via Microsoft NetMeeting).

• All types of conventional electron microscopy including routine plastic embedding prep.; negative staining; metal shadowing; "Kleinschmidt" (RNA/DNA prep.).

• All aspects of Immunocytochemistry including immunofluorescence; immunogold; and immunoperoxidase on pre- and post-embedded samples (i.e. cryosections and hydrophilic embedment).

• Contrast enhancement of LM specimens via DIC (Nomarski); phase; and digital techniques.

• Epifluorescence utilizing multiple excitation and/or emission wavelengths with high temporal resolution for ratio imaging and other applications.

• Ultra-fast simultaneous two-channel TIRF/fluorescence acquisition with dual Cascade II 512 EM cameras.

• Ultra-low light level video microscopy.

• Multi-probe detection utilizing deconvolution and confocal microscopy.

• 3-D and 4-D imaging.

• Numerous digital image enhancement/analysis techniques including point-spread deconvolution, 2-D Fourier filtering, motion analysis, sterometry, and ratio imaging.

• Microspectrophotometry.

• Cell ablation, and Fluorescence Recovery After Photobleaching (FRAP).

• Photoactivation of Intracellular Probes.

• Fluorescence Resonance Energy Transfer (FRET) acceptor photobleaching and sensitized emmission.

• In vitro and in vivo Fluorescence Correlation Spectroscopy (FCS), with single molecule detection, auto-correlation, and cross-correlation.

 

 

 

 

 

 

 

 

 

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