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INSTRUMENTATION & EQUIPMENT

The Integrated Imaging Center has the necessary instrumentation (hardware and software) required for most basic and advanced LM/EM techniques. These include:

• FEI Tecnai 12 TWIN equipped with 16 bit 2K x2K FEI Eagle bottom mount camera; and SIS Megaview III wide-angle camera (acquired with funds from NSF DBI award # 0099706, McCaffery PI; and NIH SIG award # 1S10RR023454-01, McCaffery PI)

• Philips EM 420 TEM equipped with SIS Megaview III CCD digital camera (acquired in part with funds from NIH R01GM060979-06, Wendland PI)

• FEI Quanta 200 Environmental SEM (acquired with funds from HHMI undergraduate education grant)

• 2 LEICA Ultracut UCT Ultramicrotomes/FCS Cryostages (acquired in part with funds from NSF DBI award # 0099706, McCaffery PI)

• Deltavision Deconvolving LM;

• Zeiss LSM 510 Meta Confocal with motorized stages and 405nm laser (acquired in partwith funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

• Zeiss LSM 510 VIS Confocal with Confocor 3 FCS (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

• 3-I Marianas Live Cell Imaging Workstation equipped with dual Cascade II 512 EM cameras for simultaneous 2-channel acquistion and TIRF(acquired with funds from NIH SIG award #1S10RR022588-01, McCaffery PI)

• Zeiss Axiovert inverted LM equipped with excitation and emission filter wheels; Cooke and I-Pentamax 512 digital cameras;also equipped for microinjection and micromanipulation (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

• Zeiss Axioplan upright LM equipped with a CoolSnap digital monochrome camera (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI; and NIH R01GM060979-06, Wendland PI)

• Typhoon 9410 Variable Mode Imager capable of storage phosphor, chemiluminescence, and fluorescence (acquired with funds from NIH SIG award 1S10RR021023-01, McCaffery PI)

• Avestin EmulsiFlex C3 (acquired in part with funds from NIH R01GM060979-06, Wendland PI)

• Eppendorf Microijector 5170/Micromanipulator 5242;

• Bioptech FCS-2 Environmental Stage (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

• Instec 0°-60°C stage (peltier modulated) (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

• Quantum Snap! Servers-3.0 Tbytes(acquired in part with funds from NIH SIG awards 1S10RR021023-01, McCaffery PI; and 1S10RR019409-01, McCaffery PI )

• Remote SGI, Sun, and Mac/PC post-acquistion image processing/analysis workstations;

• AGFA T2500 Multi-purpose/Multi-format color film scanner (for both reflective and transmitted scanning with up to 4000 dpi resolution).