Cell, Molecular, Developmental Biology & Biophysics

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"Quantitative Bioscience for the 21st Century."

 

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Cell Biology Research

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The oculocerebrorenal syndrome of Lowe (OCRL) is an X-linked disorder characterized by congenital cataracts, renal tubular dysfunction and neurological deficits. Lowe syndrome is due to a deficiency of phosphatidylinositol 4,5-bisphosphate (PIP2) 5-phosphatase. This results in higher than normal levels of PIP2 which in turn affect actin polymerization The Lowe syndrome fibroblasts shown have been transfected with a GFP-tagged pleckstrin homology domain (PH-GFP) to sequester PIP2 and bring its levels towards normal. Red, actin, green, PH domain, blue, counterstain.Modern Cell Biology is a highly integrative discipline in which a wide array of methods and experimental systems are used to discover the molecular bases of the fundamental cellular behaviors that are critical to the survival of all cells and organisms. In the CMDB program, we provide students with the opportunity to tackle questions at the forefront of the field in the areas of membrane trafficking, the cell cycle, nuclear organization, cellular motility and signaling, in systems as diverse as cultured cells, mice, Drosophila and yeast. Our trainees learn to approach these problems using biochemical, genetic, and molecular biological methods, in conjunction with the cutting edge microscopy methods that have historically been the hallmark of devotees of the cell (Integrated Imaging Center).

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A cytolytic T cell, labeled in red for actin is conjugated with a target cell which has been transfected with a GFP-tagged pleckstrin homology domain (PH-GFP). The PH domain sequesters the signaling lipid PI(4,5)P2, resulting in remodeling of the membrane skeleton. (see J. Kwik et. Al. Proc. Natl. Acad. Sci. USA 100: 13964-13969, 2003). Xin Chen Assistant Professor, Department of Biology
Epigenetic regulation of Drosophila germ cell differentiation from a stem cell lineage.

Kyle Cunningham Associate Professor, Department of Biology
Calcium signaling and mechanisms of gene expression in yeast.

Michael Edidin Professor, Department of Biology
Membrane organization and dynamics; immunology.

Joseph Gall Adjunct Professor, Department of Biology
Staff Member, Carnegie Institution of Washington
Chromosome structure and function; nucleic acids in development.

Samer Hattar Assistant Professor, Department of Biology
Light reception for non-image detection: role of rods, cones and the new photoreceptors (melanopsin-containing retinal ganglion cells).

Andrew Hoyt Professor, Department of Biology
Yeast chromosome segregation with emphasis on mitotic motor proteins; cell cycle regulation.

Douglas Koshland Adjunct Professor, Department of Biology
Associate Investigator, HHMI
Staff Member, Carnegie Institution of Washington and HHMI
Biochemical and genetic analysis of chromosome structure/folding; implications for chromosome evolution and genome stability.

Rejji Kuruvilla Assistant Professor, Department of Biology
Control of neuronal development by target-derived neurotrophins.

The oculocerebrorenal syndrome of Lowe (OCRL) is an X-linked disorder characterized by congenital cataracts, renal tubular dysfunction and neurological deficits. Lowe syndrome is due to a deficiency of phosphatidylinositol 4,5-bisphosphate (PIP2) 5-phosphatase. This results in higher than normal levels of PIP2 which in turn affect actin polymerization The Lowe syndrome fibroblasts shown have been transfected with a GFP-tagged pleckstrin homology domain (PH-GFP) to sequester PIP2 and bring its levels towards normal. Red, actin, green, PH domain, blue, counterstain. Trina Schroer Professor, Department of Biology
Microtubule-based motor enzymes and their roles in membrane traffic.

Mark Van Doren Assistant Professor, Department of Biology
Primordial germ cell development and migration.

Beverly Wendland Associate Professor, Department of Biology
Molecular mechanisms of endocytosis in yeast and mammalian cells.

Haiqing Zhao Assistant Professor, Department of Biology
Molecular and cellular mechanisms underlying the development and function of olfactory sensory neurons.

Yixian Zheng Adjunct Associate Professor, Department of Biology
Assistant Investigator, HHMI
Staff Member, Carnegie Institution of Washington
Biochemical, structural, and genetic analysis of microtubule organization and cell division.

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