Ostermeier, M., S. Lutz. and S. J. Benkovic "Generation of protein fragment libraries by incremental truncation." In: Golemis, E.A. (ed) Protein-Protein Interactions: A Molecular Cloning Manual. Cold Spring Harbor Laboratory Press (Plainview, NY, 2002). CORRECTIONS p. 517 step 21. replace "Klenow buffer" with "Klenow mix" both times it appears. p. 517 step 22. deleted the word "buffer" p. 519 step 16 . replace "Klenow buffer" with "Klenow mix" p. 519 step 17. deleted the word "buffer" p. 520 step 16. replace "Klenow buffer" with "Klenow mix" p. 520 step 17. deleted the word "buffer" UPDATE. p.516 step 9. We have obtained improved results by reducing the amount of mung bean nuclease to 3 units in this step. |