This collection of protocols comes from our lab as well as others but all are used by us. We hope you find them useful... and of course we do like feedback so drop us a line if you have any useful suggestions.
Cell culture
Primary Chick retinal cultures
Retinal organ culture
 
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Histology
Histologic stains - H&E
 
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Laser Capture Microdissection
LCM setup -including tissue processing.
RNA isolation -Qiagen RNA microkit.
aRNA amplification -Epicentre RNA amplication kit.

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Molecular
RNA isolation
cDNA synthesis
single cell global amplification
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In situ -solutions
In situ - probe synthesis
In situ - on cryosections