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Chroma Technology (filter/spectra information)
System Fluorophore (recommendations)
Fluorophores/Dyes
| Fluorophore/Dye Spectra | ||||
| Name | Excitation 1 |
Excitation 2 |
Emission 1 |
Emission 2 |
| 4 Metylumbelliferon | 360 |
450 |
||
| 6 Aminoquinolin | 360 |
443 |
||
| 7 Amino Actinomycin D | 546 |
647 |
||
| Acridingelb | 470 |
550 |
||
| Acridine orange (+ DNA) | 502 |
526 |
||
| Acridine orange (+RNA) | 460 |
650 |
||
| Acriflavin-Feulgen | 480 |
550-600 |
||
| Alexa 350 | 343 |
440 |
||
| Alexa 430 | 430 |
540 |
||
| Alexa 488 | 491 |
515 |
||
| Alexa 532 | 523 |
548 |
||
| Alexa 546 | 553 |
569 |
||
| Alexa 568 | 573 |
596 |
||
| Alexa 594 | 585 |
610 |
||
| Alizarinkcomplexion | 530-560 |
580 |
||
| Allophycocyannin | 630 |
660 |
||
| AMCA | 345 |
440-456 |
||
| Amino-Methylcumarin | 354 |
441 |
||
| Atebrin | 436 |
490 |
||
| Auramin | 460 |
550 |
||
| Auraphospin | 450-490 |
515 |
||
| B-Phycoerythrin | 546 |
565 |
575 |
565 |
| BAO | 365 |
395 |
||
| BCECF | 430 |
480 |
520 |
480 |
| Berberinsulfat | 430 |
550 |
||
| BFP (Blue-shifted/Y66H) | 380 |
440 |
||
| BFP2 | 380 |
440 |
||
| Blue Fluospheres | 360 |
415 |
||
| BOBO-1 | 462 |
481 |
||
| BOBO-3 | 570 |
602 |
||
| BODIPY | 503 |
512 |
||
| BODIPY 581/591 phalloidin | 584 |
592 |
||
| C-Phycocyanin | 605 |
645 |
||
| Calcein | 495 |
520-550 |
||
| Calcein Blue | 360 |
440-450 |
||
| Calcium Crimson Indicator | 590 |
615 |
||
| Calcium Green | 506 |
533 |
||
| Calcium Orange | 554 |
576 |
||
| Calcofluor White | 440 |
510 |
||
| Cascade Blue | 400 |
420 |
||
| Catecholamine | 410 |
470 |
||
| CFP(“cyan”/Y66W) | 433 |
453 |
475 |
453 |
| Chinacrin | 450-490 |
515 |
||
| Chromomycin/Mitramycin | 436-460 |
470 |
||
| Coriphosphin | 460 |
575 |
||
| CPM | 385 |
471 |
||
| Crimson Fluospheres | 625 |
645 |
||
| CTC(5-cyano2,3ditolylTetrazolium Chlorid) | 602 |
111 |
||
| Cyanine 3 Cy 3 | 553 |
575 |
||
| Cyanine 5 Cy 5 | 651 |
674 |
||
| Cyanine 7 Cy 7 | 710 |
805 |
||
| Dansylchlorid | 380 |
475 |
||
| DAO DIAMINO Phenyl Oxyd | 470 |
610 |
||
| DAPI + DNA | 359 |
461 |
||
| Dark Red FluoSpheres | 660 |
680 |
||
| dE GFP | 488 |
509 |
||
| Diaminonaphtylsulfonsaure | 340 |
525 |
||
| DiBac4(Dibutylbarbitursaure Trimethinoxonol) | 493 |
516 |
||
| DIDS Thiocyanatostilbene | 342 |
418 |
||
| DilC3/4/12/22 Indocarbocyanine | 540-560 |
556-575 |
||
| DiOC7/16/2 Carbocyanine | 550 |
580 |
||
| DiSC1/2/6 Thiocarbocyanine | 559 |
585 |
||
| Dopamin | 340 |
490-520 |
||
| DTAF | 495 |
528 |
||
| EBFP | 380 |
440 |
||
| ECFP | 443 |
453 |
475 |
453 |
| EGFP | 488 |
509 |
||
| EOSIN | 524 |
544 |
||
| Ethidium bromide (+DNA) | 510 |
595 |
||
| Euchrysin | 430 |
540 |
||
| Evans blue | 550 |
611 |
||
| EYFP | 514 |
488 |
527 |
488 |
| Fuelgen | 480 |
560 |
||
| FIF-Falk | ||||
| Fluo-3 | 480 |
520 |
||
| Fluorescamin FLURAM | ||||
| Fluoresceindiacetat FDA | 499 |
|||
| Fluoresceinisothiocyanat FITC | 490 |
525 |
||
| Fluorogold | 350-395 |
530-600 |
||
| FURA-2 | 340 |
363 |
500 |
363 |
| Generic blue/green combination | ||||
| GFP (AAV) | 395 |
470 |
510 |
470 |
| GFP (ASV) | 395 |
470 |
510 |
470 |
| GFP (LVA) | 395 |
470 |
510 |
470 |
| GFP-S65T (red shifted) | 488 |
509 |
||
| GFP mut3.1 | 395 |
470 |
510 |
470 |
| GFP UV | 360-400 |
510 |
||
| Haematoporphyrin | 530-560 |
580 |
||
| Hoechst 34580(Bisbenzimid) | 392 |
440 |
||
| Hoechst 33342(Bisbenzimid) | 352 |
461 |
||
| Indo-1 | 360 |
410 |
||
| Lissamin-Rodamine B | 574 |
602 |
||
| Lucifer Yellow | 428 |
540 |
||
| Magdalrot | 524 |
600 |
||
| Mepacrin | ||||
| Merocyanin | 555 |
578 |
||
| Methylgrun/Pyronin Stilben | 364 |
395 |
||
| Mithramycin | 420 |
575 |
||
| NBD-Amine | 460-485 |
534-542 |
||
| NBD-Chlorid | 480 |
510-545 |
||
| Nil Rot | 549 |
628 |
||
| Noradrenalin | 340 |
490-520 |
||
| Olivomycin | 340-480 |
470-630 |
||
| Oxytetracyclin | ||||
| Pararosanilin-Feulgen | 560 |
625 |
||
| PBFI | 340 |
380 |
557 |
380 |
| Phosphin 3R | 465 |
565 |
||
| POPO-1 | 434 |
456 |
||
| POPO-3 | 534 |
570 |
||
| Primulin | 410 |
450 |
||
| Propidium iodide | 536 |
617 |
||
| Pyren | 343 |
380-400 |
||
| Pyronin | 490-580 |
530-610 |
||
| QUIN 2 | 340 |
365 |
490 |
365 |
| Quinacrine mustard | 423 |
510 |
||
| R-Phycoerythrin | 480-550 |
578 |
||
| R-Phycolrythrin | 565 |
575 |
||
| Red Fluorospheres | 580 |
605 |
||
| Resorufin | 571 |
585 |
||
| Rhodamine | 540-507 |
529 |
||
| Rhodamine 123 | 540-560 |
580 |
||
| Rhodamine phalloidin | 550 |
575 |
||
| Saurefuchsin, Fuchsinsaeure | 540 |
630 |
||
| SBFI | 340 |
380 |
420 |
380 |
| Sertonin | 365 |
520 |
||
| SNARF | 480 |
600 |
||
| Stilben SITS, SITA | 365 |
460 |
||
| Sulfaflavin | 380-470 |
470-580 |
||
| Tetracycline | 390 |
560 |
||
| Tetrametylrhodamine | 540 |
566 |
||
| Texas Red | 595 |
620 |
||
| Thiazinrot | 510 |
580 |
||
| Thioflavin S | 430 |
550 |
||
| TOTO-1 + DNA | 509 |
533 |
||
| TOTO-3 + DNA | 642 |
661 |
||
| TRITC | 520-554 |
580 |
||
| wtGFP | 395 |
470 |
510 |
470 |
| XRITC | 572 |
596 |
||
| Yellow-Green Fluospheres | 505 |
515 |
||
| YFP(Au yellow‚ Au T203Y) | 513 |
488 |
527 |
488 |
| YOYO-1 | 491 |
509 |
||
| YOYO-3 | 612 |
631 |
||